FLUORESCENCE HISTOCHEMICAL AND CHEMICAL ANALYSES OF CATECHOLAMINES IN BULLFROG ADRENALS
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概要
- 論文の詳細を見る
Formaldehyde-induced fluorescence microscopy, microspectrofluorimetry and chemical assay were applied to bullfrog adrenals. Chemical determination showed that bullfrog adrenals contain excessively high amounts of noradrenaline and adrenaline and only a small amount of dopamine. Two cell types (chromaffin cells showing an orange fluorescence and acidophilic summer cells showing a green fluorescence) were observed in sections of bullfrog adrenals treated with formaldehyde vapor of 60-75% humidity equilibration at 80℃ for 1 hour. The two cell types showed excitation/emissiom maxima at 410-420/510-560 mμ and at 410-420/500 mμ, respectively. When treated with formaldehyde vapor at 50℃ for 1 hour, chromaffin cells were divided into two types (one type showing an orange fluorescence and the other showing a green fluorescence). The orange and the green fluorescing chromaffin cells showed excitation/emission maxima at 420/470 mμ and at 410-420/460-470 mμ, respectively. A green fluorescing ganglion cell also was found. This data indicates the presence of four cell types containing catecholamines (two types of chromaffin cells containing noradrenaline and adrenaline, acidophilic summer cells containing adrenaline and ganglion cells probably containing adrenaline) in bullfrog adrenals.
- 日本組織細胞化学会の論文
著者
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Ito Ryozo
Institute of Geosciences, Shizuoka University
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Onogi Kenji
Instruments Designing Department Nippon Kogaku K. K.
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Kojima Hideki
Institute Of Brain Diseases Kurume University School Of Medicine
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ANRAKU Shigemi
Institute of Brain Diseases, Kurume University School of Medicine
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KUWAHARA MITSUKO
Institute of Brain Diseases, Kurume University School of Medicine
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ONOGI KENJI
Institute of Brain Diseases, Kurume University School of Medicine
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Anraku Shigemi
Institute Of Brain Diseases Kurume University School Of Medicine
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Kuwahara Mitsuko
Institute Of Brain Diseases Kurume University School Of Medicine:designing Department Instrument Div
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KOJIMA HIDEKI
Institute of Brain Diseases
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ANRAKU SHIGEMI
Institute of Brain Diseases
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