HISTOCHEMICAL STUDY ON THE INTRAVITAL REDUCTION POTENCY OF COENZYME Q IN THE RESPIRATORY CHAIN OF THE HAIR CELLS OF THE SPIRAL ORGAN IN NORMAL GUINEA PIGS AND ITS ALTERATION IN ACUTE STARVATION AND VITAMIN B_1 DEFICIENCY
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概要
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The present study revealed that the intravital reduction of Nitro-BT can occur in the absence of any exogenous substrate in the living hair cells of the cochlea in nembutal anaesthetized guinea pigs. Electron transfer inhibitors, such as amytal and malonate considerably impeded the intravital reduction of Nitro-BT. The reduction of Nitro-BT was completely blocked after heating at 100℃ for 15 minutes and cooling at -20℃ for 30 minutes. The reduction of Nitro-BT continued to some extent in the hair cells left at room temperature at least for 60 minutes after sacrification. Acute complete starvation caused considerable decrease in the intravital reduction of Nitro-BT and vitamin B_1 deficiency did rather slightly. The intravital reduction of Nitro-BT seemed to be due to the intravital multi-enzymatic tetrazolium reducing reaction. The present study suggested that the intravital enzymatic tetrazolium reducing reaction may depend on the amount of electrons which are transferred by CoQ in the respiratory chain to Nitro-BT. The amount of electrons transferred by CoQ to Nitro-BT was considered the intravital reduction potency of CoQ. The intravital reduction potency of CoQ seemed to depend on cellular metabolism and its rate in the hair cells.
- 日本組織細胞化学会の論文
著者
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SATO Kiichi
Department of Applied Chemistry, School of Engineering, The University of Tokyo
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Sato Kiichi
Departntent Of Pathology Institute For The Deaf Tokyo Medical And Dental University
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Sato Kiichi
Department Of Applied Biological Chemistry School Of Agricultural And Life Science The University Of
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AKIYOSHI Masatoyo
Department of Pathology, Institute for the Deaf, Tokyo Medical and Dental University
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NAKADA Hozumi
Departntent of Pathology, Institute for the Deaf Tokyo Medical and Dental University
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Nakada Hozumi
Department Of Pathology Medical Res. Institute Tokyo Med. & Dent. Univ.
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Akiyoshi Masatoyo
Department Of Pathology Medical Res. Institute Tokyo Med. & Dent. Univ.
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Akiyoshi Masatoyo
Department Of Pathology Institute For The Deaf Tokyo Medical And Dental University
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