腎保存に関する基礎的研究 : 特に腎エネルギー代謝を中心として

概要

論文の詳細を見る
The renal preservation has become one of the most important problems in human renal transplantation. The purpose of this paper is to find a better way to preserve the kidney, by evaluating energy metabolism and tissue respiration of the preserved canine kidneys. Hurbert and Potter's method (with column chromatography using Dowex-1 x-8 resin) was employed to the slices of the kidney to determine the renal adenosine nucleotides (ATP). Oxygen consumption (QO_2) was also determined in the cortex of the sliced kidney with Warburg manometer. Both parameters, ATP content and QO_2, were the excellent methods to examine the viability of the preserved kidney. They declined gradually after storage for six hours and decreased markedly thereafter. As far as the preservation methods were concerned, hypothermic (4℃) saline immersion under hyperbaric oxygen atomosphere as well as the hypothermic perfusion with canine plasma plus 10% dextran (1:1) under hyperbaric oxygen atomosphere (3 atomospheres) was beneficial. Metabolic benefits were not seen after the storage by simple preservation at room temperature, hypothermic immersion alone, preservation under hyperbaric oxygen atomosphere, or the combination of the two latters. When 10^<-4> M of inosine was mixed to the perfusate composed of canine plasma plus 10% dextran, ATP content in the kidney was significantly increased, thus inosine was proved efficacious to preserve the canine kidneys.
社団法人日本泌尿器科学会の論文

腎保存に関する基礎的研究 : 特に腎エネルギー代謝を中心として

スポンサーリンク

概要

著者

関連論文

スポンサーリンク