ヒト尿中線維素溶解酵素系 : 附フィブリン平板溶媒の吟味

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Fibrinolytic enzyme system in fresh urine and acid treated urine was examined on fibrin plates, where various kinds of solvents of fibrinogen and thrombin was used. Acid treated urine was made as follows, the pH of fresh urine was adjusted to 2 with 1 N HCl, and neutralized with I N NaOH, after standing for 30 minutes. 1) As the solvent of fibrinogen-and thrombin-solution, borate saline buffer was most suitable for making fibrin plates to estimate fibrinolytic activity of urine, and veronal buffer was available when the activity of urine sample was weak. Physiologic saline was in use, when the activity was estimated within 18 hours. 2) It is known that there are urokinase, uropepsin, trypsin inhibitor and thromboplastin-like sub-stances, as substances related with fibrinolysin in urine. Nevertheless, fresh urine (non treated urine) was available in the examination of fibrinolytic activity in urine clinically. 3) As the control enzyme, 10 u/ml of UK was available in order to determine the fibrinolytic activity in urine on fibrin plates. The activity of UK did not decrease after keeping for 21 days in 20℃. When the lysed area was determined after incubation for 18 hours, 100 u/ml trypsin, a rather stable enzyme, was available for the purpose. 4) By the acidification of urine, even after neutralization, the fibrinolytic activity of urine was extremely decreased. In the process of acid treatment, pepsinogen in urine might be converted to pepsin and then the enzyme might destroy UK. Despite of the destruction of UK, the lysed area produced by acid treated urine plus UK or acid treated urine plus trypsin was always larger than that produced by the same units of UK or trypsin separately. This result suggests that there exists a pre-enzyme which is stable by acidification. 5) The lysed area produced by fresh urine plus trypsin was smaller than that produced by only fresh urine. This implies that there exists the inhibitor of fibrinolysis in urine. But on the contrary, acid treated urine plus trypsin showed stronger fibrinolytic activity than that of only trypsin or acid treated urine. This suggests the possibility that there is a pre-enzyme which will be activated by trypsin in urine.
社団法人日本泌尿器科学会の論文

ヒト尿中線維素溶解酵素系 : 附フィブリン平板溶媒の吟味

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ヒト尿中線維素溶解酵素系 : 附 フィブリン平板溶媒の吟味

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ヒト尿中線維素溶解酵素系 : 附フィブリン平板溶媒の吟味