泌尿***悪性腫瘍の制癌剤多剤併用療法に関する研究 : 第1報 : 全血培養によるリンパ球幼若化測定法についての検討

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The rate of lymphocyte blastoid transformation (LBT) is an effective measure of the immune status of cancer patients. Conventional techniques of measuring the LBT rate have depended on isolated cultures of purified lymphocytes. Such data show, statistically, that a decrease in the LBT rate occurs reflecting the extent of progression of the tumor. The individual results, however, show a comparatively wide scatter. The present authors, therefore, in cognizance of the changes that occur in the LBT rate (especially during anti-cancer chemotherapy) introduced the Pellegrino method of whole blood culture to measure the rate of LBT. This was than used in the clinical assessment of the value of the LBT rate as an index of the therapeutic effect of anti-tumor drugs. The method showed a significant correlation with the results of isolated culture of lymphocytes (r=0.9078, p<0.01) and, since less than one ml of blood is needed per test, had the added advantage of being a simple and convenient operation. The present experiment was an investigation of the applicability of this method to rats (body weight approximately 180g). Consistent results were obtained under conditions of a PHA-P concentration of 0.2%, the blood volume of each test being 0.1ml. Best results were obtained, with a four-day culture period, <125>^I iode-5-deoxyuridine (<125>^IUdR) was best added six hours prior to the proposed termination of culture, 0.5μCi of <125>^IUdR proving adequate for the purpose. Coincidentally, it was shown that 1 ml/week of blood loss is no burden on the rat and that this species is useful in monitoring the changes occurring in the rate of LBT with the passage of time.
社団法人日本泌尿器科学会の論文

泌尿***悪性腫瘍の制癌剤多剤併用療法に関する研究 : 第1報 : 全血培養によるリンパ球幼若化測定法についての検討

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