Candida albicans の germ tube および菌糸形成における thiazolidine-4-carboxylic acid の影響

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Solid media and culture conditions for mycelial (M) growth were investigated for the purpose of defining the mechanism of the yeast (Y)-M transition in Candida albicans (C. albicans) ATCC 1002 strain. Germ tube (GT) formation media were prepared based on results obtained by using solid media. The effect of thiazolidine-4-carboxylic acid (thioproline) (selected as one of the growth media components) on germination in C. albicans was studied. The results obtained were as follows. 1. When each amino acid was added to agar media composed of 10^<-2>% MgCl_2 and 10^<-2>% NaH_2PO_4, L-proline was the only amino acid to stimulate good M growth. 2. A gaseous phase containing CO_2 over 1% was necessary to stimulate M growth on agar media composed of 10^<-2>% L-proline, 10^<-2>% MgCl_2 and 10^<-2>% NaH_2PO_4. 3. When the organism was cultivated using the candle jar method on the media containing 10^<-2>% L-proline and one of the following : 10^<-2>% thioproline, 10^<-2>% L-cysteine, 10^<-1>% L-methionine or 10^<-1>% MgCl_2, good M growth was observed. The best M growth was obtained on the plate containing thioproline and L-proline. 4. Based on the results of the experiments using solid media, a TPC medium composed of 10^<-2>% thioproline (T), 10^<-2>% L-proline (P) and 10^<-2>% NaHCO_3 (C) was devised for GT formation. 74% of the cells in TPC medium developed GTs and 2% of those in PC medium (TPC medium minus thioproline) developed GTs. 5. The effective inoculum sizes for GT formation in TPC medium were under 10^5 CFU/ml. When organisms in excess of 10^6 CFU/ml were inoculated in the medium, only 20% of the organism formed GTs. 6. The following additives put on the TPC medium inoculated with 10^6 CFU/ml caused over 90% of the cells to develope GTs : 10^<-2>% sodium linoleic acid, 10^<-2>% sodium oleic acid, 10^<-2>% linolenic acid, or 1% tween 80. About 90% of the cells formed GTs in TPC medium supplemented with 10^<-2>% sodium linoleic acid (L) ; in LPC medium 2% of the cells formed GTs. 7. Substitutes for thioproline in LTPC medium were investigated. Sulfur containing amino acids (L-methionine, L-cysteine, L-cystine), metabolic intermediates, taurine, and some inorganic sulfur compounds (except Cu, Zn, Mn compounds) were effective. 8. Thioproline concentrations that accelerated GT formation in the LTPC medium ranged from 3×10^<-5>% to 3×10^<-2>%. The best GT formation was obtained in concentrations of 3×10^<-3>% to 10^<-2>%. About 90% of the cells formed GTs. However GT formation was inhibited by thioproline at 10^<-1>%. 9. The inhibition by 10^<-1>% thioproline was bacteriostatic. 10. The cells' uptake of L-proline in the LTPC medium was accelerated by 10^<-3>% thioproline. The uptake reached a maximum at 10^<-2>%, and was inhibited at 10^<-1>%.
九州歯科学会の論文
1985-12-25

Candida albicans の germ tube および菌糸形成における thiazolidine-4-carboxylic acid の影響

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