P-12 歯科材料の簡易・高感受性細胞毒性試験法の開発(2)

概要

論文の詳細を見る
A transformed human dental pulp cell line(LSC)was tested for the sensitivity to dental composite resin with MTT assay and Neutral Red(NR)assay.L-929 cells were used as a reference cell line.The sensitivity between two types of cells and between the two test assays were investigated.According to IC50, there were remarkable statistic significant difference with two kinds of cell lines and test assays(P<0.005).The MTT assay showed lower sensitivity than NR assay in L-929 cells, and LSC cells showed less sensitivity than L-929 cells in NR assay.It has demonstrated that the differential method or cell line used may have some effects on final results.Both assays are economical, reproducible and effective methods in detecting the cytotoxicity of biomaterials, MTT assay may be more suitable for screening a wide large of unknown materials.
日本歯科理工学会の論文
1995-03-31