放線菌による新規含リン除草剤ビアラホスの工業的生産

概要

論文の詳細を見る
A breeding study was performed from the viewpoint of biochemical engineering to obtain strains of microorganisms which produce bialaphos (a herbicide) efficiently (high productivity and high yield) from inexpensive substrates under low oxygen concentration conditions, with good filtration efficiency and easy downstream treatment. The strain obtained was found to produce about six-hundred times higher product concentration than the wild strain. With this new strain, it was also possible to use an inexpensive carbon source and to produce bialaphos under low oxygen concentration conditions, with good filtration efficiency. The activities of the tricarboxylic acid cycle enzymes of the new strain were lower than those of the wild strain. while the activities of the glyoxylic acid cycle enzymes of the new strain were higher than those of the wild strain. When the scale-up was based on power consumption (for agitation) per unit volume of the reactor, bialaphos production in the 300 kl fermentor was 50% of that obtained with the 3l jar fermentor. When the scale-up was based on the DO concentration and the DO concentration at the middle of the 300 kl fermentor was controlled at 0.5 ppm (optimum DO concentration for bialaphos production in the 3l fermentor), bialaphos production was increased to 85% of that obtained with the 3l jar fermentor. Taking the DO concentration gradient in the 300kl fermentor into consideration, the DO concentration at the bottom of the fermentor was maintained at 0.5 ppm. This resulted in a successful scale-up and bialaphos production increased to 96% of the maximum production in the 3l jar fermentor. In bialaphos fermentation, production of bialaphos is greatly reduced when the partial pressure of oxygen is increased. Methylation at the final step of the biosynthetic process of bialaphos was not induced under high partial pressure, resulting in a low bialaphos concentration due to the accumulation of the demethylated derivatives.
社団法人日本生物工学会の論文
1995-09-25

放線菌による新規含リン除草剤ビアラホスの工業的生産

スポンサーリンク

概要

著者

関連論文

スポンサーリンク