耐熱性凝集性酵母を用いた繰返し回分発酵の安定性に及ぼす通気および温度の影響

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The effects of aeration and fermentation temperature on the stability of repeated-batch fermentation using a thermotolerant flocculating yeast, Saccharomyces cerevisiae KF-7,were investigated. Repeated-batch fermentation tests were carried out with a non-sterilized molasses medium containing 20% (w/v) sugar. Under continuous aeration, condition during ethanol fermentation the ethanol productivities were almost constant, being 8.5,7.7,and 7.7 g/l・h at 30,33 and 35℃, respectively, and the conversion ratio of the sugar consumed to the ethanol produced was more than 0.45,a value which corresponds to over 88% (hereafter called the ethanol yield) of the theoretical value of 0.51 at each temperature. However, the number of viable cells decreased with stepwise increases in temperature. At 33℃, the aeration time was decreased stepwisely. The productivity and viable cells were kept constant, at about 7.6g/l・h and 4.0×10^8 cells/ml even when aeration was applied only for the first 5 h of each fermentation. However, under non-aeration conditions, they decreased to 5.1g/l・h and 1.3×10^8 cells/ml, respectively, but the fermentation could be repeated stably. At 35℃, the fermentation could not be conducted stably when the aeration conditions were changed from continuous aeration to aeration for the first 5h. During the 80 batches of repeated-batch fermentation, except when aeration was conducted the first 5h at 35℃, the ethanol yield was maintained stably at more than 90%.
社団法人日本生物工学会の論文
1995-03-25

耐熱性凝集性酵母を用いた繰返し回分発酵の安定性に及ぼす通気および温度の影響

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