有機物中の微量リンの定量

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Less than 10μg or 10 ppm phosphorus in organic materials was determined by a low-temperature ashing-molybdenum blue spectrophotometry. After a sample had been treated by low-temperature asher, the residue was taken up and fumed with 15 cm^3 of 30 (w/w)% perchloric acid. After cooling the perchloric acid solution was transferred to a 300 cm^3 separatory funnel, to which 10 cm^3 of 5 (w/v)% ammonium molybdate was added and the mixture was diluted to 100 cm^3 with water. Phosphorus was extracted into 10cm^3 of butyl acetate by shaking the mixture for 1 min. The organic phase was transferred to a 25 cm^3 volumetric flask and was diluted with ethanol to the mark. After the addition of 0.2cm^3 of 5 (w/v)% tin(II) chloride, the absorbance was measured at a wavelength of 725 nm against water. Results on the determination of phosphorus in rice and filter papers were quoted and compared with those by other ashing methods. Recoveries were checked with tetraphenyl-phosphonium chloride, yielding 95.4% as an average.
社団法人日本分析化学会の論文
1979-03-05