化学発光反応を用いる微量タンパク質のフローインジェクション分析法の改良
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概要
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著者らが開発した微量タンパク質のFIA法において,装置を単純化すると共に,操作法を迅速,容易かつ簡便化した.各試溶液の送液には,これまでの窒素ガス圧の代わりに無脈流送液ポンプを使い,分析装置を極めて簡素化できた.又各溶液は,脈流を生じることなく送液され,それぞれの流量は迅速,正確かつ低流量に調整できた.化学発光強度の測定にフローセル部及び光電子増倍管部をそれぞれ温度制御できる化学発光検出器を用いることによって,冷却チューブや恒温槽を使うことなく,容易に,安定した化学発光強度を測定できた.又,発光試薬(1,10-フェナントロリン)と金属触媒{銅(11)}をあらかじめ混合した溶液が使用できることを見いだし,送液流路数を減らすことができた.本報のFIA装置を使い,ウシ血清アルブミンを定量濃度範囲5×10^<-6>〜1×10^<-2>g dm^<-3>,検出限界値250 pg(S/N=3)で測定できた.1.0×10^<-4>g dm^<-3>ウシ血清アルブミンにおける相対標準偏差及び分析速度は,それぞれ7.4%(n=5)及び毎時12試料であった.ウシ血清アルブミンのピーク面積を1.00としたとき9種類のタンパク質の1.0×10^<-4>g dm^<-3>濃度における相対ピーク面積は0.84〜1.42であった.
- 社団法人日本分析化学会の論文
- 1989-07-05
著者
-
塚越 一彦
同志社大学理工学部化学システム創成工学科
-
原 正
同志社大学
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紀本 英志
同志社大学工学部化学工学科
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Tsukagoshi Kazuhiko
Department Of Chemical Engineering And Materials Science Faculty Of Science And Engineering Doshisha
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Tsukagoshi Kazuhiko
Dep. Of Chemical Engineering And Materials Sci. Fac. Of Engineering Doshisha Univ. Kyotanabe Kyoto 6
-
塚越 一彦
同志社大学理工学部
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