セルロース混合ポリビニルアルコールを基質とする室温りん光法におけるりん光強度再現性の向上

概要

論文の詳細を見る
The effects of the drying condition in a microwave oven and the surface form of a poly-(vinyl alcohol) substrate on the room-temperature phosphorescence (RTP) intensity and its reproducibility were investigated by monitoring the p-amino benzoic acid (PABA) RTP intensity. The position of the substrate in the microwave oven used in the drying process severely affected the RTP intensity. Reproducible and intensive values were obtainable when the substrate was dried on an activated silica-gel layer in a small crucible fixed at the center of a rotating plate in a microwave oven. The calibration curve under this condition was linear from 0.2 to 1200pmol/spot with a correlation coefficient of 0.999,and nearly intercepting the origin. Grooving the substrate surface with cross stripes before drying further improved the reproducibility of the RTP signal intensity of PABA with a relative standard deviation of 9.7%.
社団法人日本分析化学会の論文
1997-03-05