ミクロ液体クロマトグラフィー : 高速原子衝撃質量分析法によるマウス胸腺細胞に存在するジシアロガングリオシドの構造解析(<特集>質量分析法の応用技術)

概要

論文の詳細を見る
A micro method of high-performance liquid chromatography/fast atom bombardment mass spectrometry (LC/FAB/MS), which involves a micro silica gel column and a micro LC apparatus working at a flow rate of 6ml/min, has been developed for the structural characterization of mouse thymocytes. Four disialogangliosides extracted from 2.2×10^9 thymocytes were identified as [GD1c (NeuAc, NeuAc), GD1c (NeuAc, NeuGc), GD1c (NeuGc, NeuAc) and GD1c (NeuGc, NeuGc)] from the obtained mass spectra. This micro LC/FAB-MS system is useful not only for the structural charactization of neutral glycosphinglipid and monosialoanglioside mixtures, as reported previously, but also disialoganglioside mixtures prepared from a limited number of isolated cells.
社団法人日本分析化学会の論文
1996-06-05