p-ニトロベンズアルデヒド法とブロモクレゾールグリーン法を組み合わせた血清アルブミン/グロブリン比の測定法に関する検討

概要

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The determination method (NB method) for the serum albumin/globulin ratio by combining the Bromocresol Green (BCG) method for the serum albumin and the p-nitrobenzaldehyde (p-NITBA) method for the serum total globulin was compared with the conventional methods. The measurement values by the NB method correlated well with those by the BB method by combining the biuret method for the serum total protein and the BCG method(r=0.969, y=0.977x-0.151, S_yx=0.095, n=100) and with those by the GB method combining the method of Goldenberg et al and the BCG method(r=0.984, y=0.898x-0.166, S_yx=0.069, n=100). The mean value(1.31) of the NB method was lower than those of the BB method(1.49) and the GB method(1.64). The sum of the serum albumin and the serum total globulin obtained by the NB method correlated well with the serum. total protein by the biuret method(r=0.970, y=1.036x+1.87, S_yx=2.19g/1, n=100). From these results, it is thought that the NB method can be used to measure the serum albumin/globlin ratio.
社団法人日本分析化学会の論文
2002-08-05