溶媒抽出-高速液体クロマトグラフィーによる果樹葉中の微量鉛,ニッケル及び銅の同時定量

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High performance liquid chromatography (HPLC) was applied for the determination of lead, nickel, and copper in Orchard Leaves followed by solvent extraction. Ammonium pyrrolidinedithiocarbamate (APDC) was employed as the chelating agent for the above heavy metals. Orchard Lcaves (500 mg) sample was ashed at 450℃ for (15〜20) h and dissolved with 2 ml of 2 N hydrochloric acid, then the solution was put into the separatory funnel and diluted up to 50 ml with water. Ten milliliters of 1.O M ammonium citrate, dibasic solution (pH9.3) and two drops of O.1 % metacresol purple solution were added. Ammonia water (1+1) was added until purple color was developed. Then 2.O ml of O.01 MAPDC and 1.0 ml of chloroform were added accurately. After shaking the contents for 15 min, a 3μl portion of chloroform layer was injected into the HPLC column (Cosmosil 5 C_<18>, 4.6mm×150mm). The mixed solution of acetonitrile-water-O.Ol M APDC(67 : 33 : O.15) was used as the eluent. The proposed method permitted simultaneous determination of lead, nickel, and copper in Orchard Leaves. Analytical results were in good agreement with the certified values of standard sample.
社団法人日本分析化学会の論文
1983-04-05