抗生物質共存下におけるビタミン類の微生物定量法 : (V)コリスチンおよびバシトラシン共存下におけるビタミン類の定量

概要

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A microbiological assay method of vitamins in samples containing colistin and bacitracin, cyclic peptide antibiotics, was described. Antibacterial activities of these antibioties to St.faecalis, Leuc.mesenteroides P-60,L.arabinosus 17-5,L.casei and L.fermenti 36 were tested, in order to screen a species of which growth was not inhibited. St.faecalis R and L.casei were naturally resistant to colistin and not inhibited even up to 1000μg/ml of colistin. On the other hand, the growth of all these lactobacilli was inhibited in the presence of relatively low concentration of bacitracin. The bacitracin resistant strains of St.faecalis R and L.arabinosus 17-5 were isolated by successive transfer in the medium containing graded amount of bacitracin. Assay range of pantothenic and folic acid with the former and that of pantothenic acid with the latter were different from that with parent strains. In the case of resistant strains, there was observed a little shift towards high concentration of vitamins. It was shown that when the colistin was contained in the sample, pantothenic, folic and nicotinic acid were able to assay with St.faecalis R, and when the bacitracin was contained, these vitamins were determined by bacitracin resistant strains.
日本ビタミン学会の論文