ガラスキャピラリーカラムを用いるプロビタミンD及びビタミンD紫外線照射反応物のガスクロマトグラフィー

概要

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Gas-liquid chromatographic (GLC) separation of irradiated mixtures of provitamins D and vitamins D on wall coated open tubler glass capillary column (capillary column) was investigated and the results were compared with those on packed column. A glass capillary. (0.25mm×28m) was wallcoated by OV-17,whereas a packed column was made by packing a glass column (0.3×260cm) with 1.5% OV-17 on Shimalite W (80〜100 mesh). When the reaction mixtures of ergosterol, 7-dehydrocholesterol, vitamin D_2 and D_3 irradiated by the monochromatic light at 295nm were trimethylsilylated and then applied to the both GLC, the peaks obtained by the capillary column-GLC were generally sharper than those obtained by the packed column-GLC. The former's theoretical plate efficiency and resolution were better than the latter's. The peaks of suprasterol I and II unseparatable by the packed column could be separated each other by the capillary column.
日本ビタミン学会の論文
1976-04-25