各種キノコ中のビタミンD_2の同定及び定量

概要

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Vitamin D_2 in fungi cultured in rooms without solar radiation was identified and determined. The results obtained were as follows: (1) Pre-D_2 and vitamin D_2 in raw Maitake (Grifola frondosa) was identified by HPLC and GC-MS. (2) The ergosterol contents in the samples of fungi are similar to those in Shiitake (Lentinus edodes). (3) The weight ratio of pre-D_2 to vitamin D_2 in raw Maitake was about 1 : 1.9, whereas that in raw Shiitake was about 7 : 1. It was deduced from the results that vitamin D_2 in Maitake might be produced without solar radiation. (4) The assayed values of vitamin D_2 (including pre-D_2) in the samples of fungi by the HPLC method reported previously (J. Nutr. Sci. Vitaminol., 30, 11, 1984) were 88.8 IU/100 g (Maitake), 30.5 IU/100 g (Nameko, Pholiota nameko) and 28.4 IU/100 g (Hiratake, Pleurotus ostreatus), respectively. These values were much lower than those of Shiitake cultured outdoors (310 IU/100 g) but similar to those of Shiitake cultured in a vinylhouse (73 IU/100 g).
日本ビタミン学会の論文
1985-06-25