胞子形成とビオチンとの関連 : (II)胞子形成能に及ぼす細胞内ビオチンの影響

概要

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The sporulating ability of Bacillus megaterium was investigated in both biotin-dependent strain and biotin-independent strain by the active culture method, and by the technique of endotrophic sporulation. The biotin-restricted cells were prepared by incubating in the desthiobiotin-limiting medium for the biotin-dependent strain and, by incubating in the presence of ethionine for the biotin-independent strain. Under the biotin-deficient condition, the biotin-dependent strain accumulated abnormally a large amount of lipid inclusions in the cells, but the sporulation was markedly inhibited. As the amount of intracellular biotin increased, the number of spore formed increased proportionally. When the biotin-restricted cells of both strains were supplied with biotin in the sporulating-medium, the sporulating ability was markedly recovered. It is suggested that biotin is one of the factors which are related to the sporulating ability of cells in the later stages of logarithmic growth phase.
1969-05-25