ビタミンB_1の拮抗物質にかんする研究 : (XV)Kloeckera apiculataにおけるアンチビタミンB_1化合物の集積とビタミンB_1集積の阻害

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Pyrithiamine (I), when incubated with the culture medium of Kloeckera apiculata, was accumulated on cells 1.7 times as much as thiamine. The accumulated amounts of anti-thiamine compounds other than (I) were less than thiamine ; bezylimidazole-thiamine (II) : 0.67,butylimidazole-thiamine (II) : 0.45,thiamine sulfuric ester (IV) : O.09,triazino-thiamine (V) : 0.07,phenyltriazino-thiamine (VI) : 0.06,imidazole-thiamine (VII) : 0.04,desthiothiamine (VIII) : 0.02,oxythiamine (IX) : 0.02,in comparison with thiamine. When Kloeckera cells preincubated with (I) or (II), were washed and transfered to a thiamine solution, the cells released (I) or (II) gradually, as thiamine was accumulated increasingly on the cells. These anti-thiamine compounds except (IX), inhibited cell-uptake of thimine, when they were incubated with the culture of Kloeckera and thiamine. Inhibition ratios of the compounds were shown in percentage ; (II) : 87,(I) : 67,(VII) : 62,(III) : 58,(IV) : 24,(VIII) : 13,(VI) : 11,(V) : 7. From these results, it was concluded that anti-thiamine compounds of the stronger growth inhibition index to Kloeckera were more strongly accumulated on the cells and inhibited cell-uptake of thiamine more strongly at the presence of thiamine.
日本ビタミン学会の論文
1967-11-25