チオクロム生成反応 : (VII)ブロムシアンによるチオチアミンよりチオクロムの生成とpHとの関係

概要

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Thiothiamine can be almost quantitatively converted into thiochrome, when it is reacted with cyanogen bromide at the pH ranges between 3 and 12. At the higher pH than 13 thiothiamine is kept unchanged because of decomposition of cyanogen bromide by strong alkalinity. Thiochrome is formed rather slowly at room temperature all over pH ranges, since both gradual decrease of absorbancy at 320mμ (absorption maximum for thiothiamine) and increase of that at 365mμ (for thiochrome) are observed parallelly during the progress of reaction. Thiothiamine in the mixture with thiamine can be converted into thiochrome when cyanogen bromide is added in acid solution of the mixture ; after standing for 1 hour at 30℃, excess of the cyanogen bromide can be eliminated either by aeration or by addition of Na_2S_2O_3. The thiochrome is extracted by butanol after its alkalization whereas thiamine is remained unchanged. Both thiotiamine and thiamine can be transformed to thiochrome by the cyanogen bromide procedure without elimination of excessive cyanogen bromide. Thus separate estimation of both compounds in biological materials has been made possible.
日本ビタミン学会の論文
1963-08-25