DIHYDROTHIAMINEに関する研究 : (V)DIHYDROTHIAMINEの尿中代謝産物について

概要

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When 50 mg of dihydrothiamine (DHB_1) was injected to rats, only a small amount of DHB_1 was excreted in the urine and at least two metabolites were detected by its paper partition chromatography. The urine was deproteinized by lead acetate, evaporated to syrup after precipitation of lead as sulfide, and then extracted by acetone and chloroform. An oily substance was obtained from the chloroform extract and characterized as γ-aceto-γ-mercaptopropyl alcohol. The extracted residue was dissolved in water, passed through the ion-exchange resin Amberlite IR-120 column and then eluted by 2N-NH_4OH ; needle crystals of mp 264-5℃ was isolated from the eluate and identified as tri-[2-methyl-4-amino-pyrimidyl] (5)]-methyl-trimethylene-triamine. These 2 metabolites would have been derived from DHB_1 by its decomposition scheme shown in Fig. 3.
日本ビタミン学会の論文