ニンニク属植物の栄養学的価値に関する研究 : (XXVII)アリチアミンの定量法

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This paper presents procedures for the determination of thiamine allyldisulfide based on the results obtained in the previous papers. In the direct method, without adsorption procedure, thiamine allyldisulfide is first reduced with cysteine followed by determining thiamine formed, and the difference between thiamine values prior to and after the reduction, gives the value of thiamine allyldisulfide. In the adsorption method, thiamine allyldisulfide is adsorbed on Amberlite IRC-50,eluted with 1N hydrochloric acid or 2N acetic acid, reduced to thiamine, oxidized to thiochrome, and measured fluorometrically. In case of a mixture of thiamine with thiamine allyldisulfide, the former is eluted with boiling 25% potassium chloride, then the latter is eluted with 1N hydrochloric acid or 2N acetic acid, and the determination of the vitamin is performed separately.
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