Chang's Liver Cellsの増殖に及ぼすL-Methylmethionine Sulfonium ChlorideとL-メチオニンの影響

概要

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The experiments were carried out to examine the effects of L-methionine and L-methylmethionine sulfonium chloride (L-MMSC) on the biological activity of Chang's liver cells. When incubated for 72 hours in medium containing 5 mM of L-methionine, proliferation rate of cells was 72% lower than in the control cells. However, in the presence of 5 mM L-MMSC, the reduction of cellular multiplication did not appear. The incorporation of 6-^3H-thymidine into DNA was also inhibited after 3 hours incubation with 5 mM of methionine to about 86% of the control, while the same concentration of L-MMSC did not show such an inhibitory effect. The incorporation of 5-^3H-uridine into RNA in both culture was nearly equal in 3 hours incubation. The incorporation of U-^<14>C-L-leucine into protein was inhibited by 5 mM of t-methionine to about 56% of the control and by 5 mM of L-MMSC to about 70% of the control after 3 hours incubation. From these results, it was concluded that L-MMSC is less toxic than L-methionine in Chang's liver cell culture.
日本ビタミン学会の論文
1972-04-25