菌體成分に關する研究(第4報) : ペニシリン生産菌體中のステリン及びアミノ酸に就て

概要

論文の詳細を見る
(1) Crude crystal of sterols (m.p. 142°/53°) was obtained at the yield of 0.15% from the mycelium of Penicillium chrysogenum Q-176 grown in submerged culture.(2) By fractionation the following four sterols were isolated in pure state from the crude crystal.i. Ergosterol m. p. 163. [α]^<15>_D= -122.8° acetate m. p. 171.5° [α]^<15>_D= -82.2°ii. Fungisterol C_<28>H_<48>O・H_2O m. p. 147.5° [α]^<15>_D= -20.9° acetate m. p. 158° [α]^<15>_D= -14.5iii. Ascosterol C_<28>H_<48>O・H_2O m. p. 141° [α]^<15>_D= +42.7° acetate m. p. 153.5° [α]^<15>_D= +34°iv. Sterol 205 C_<28>H_<48>O・H_2O m. p. 205°/7° [α]^<15>_D= -29.1° Sterol 205 scems to be Epi-dihydro-ergosterol or γ-Dihydro-ergosterol.3. In the hydrolytic decomposition products of the mycelium fourteen amino-acids (leucine, isoleucine, tyrosine, valine, arginine, threonine, lysine, histidine, proline, alanine, glycine, serine, glutamic acid and hydroxyglutamic acid) were qualitatively determined by the partition paper chromatography. Besides them the presence of tryptophane also in the mycelium was proved by ADAM-KIEWIIZ's reaction.
社団法人日本生物工学会の論文
1950-05-15