植物細胞の高濃度細胞培養における培養工学的問題

概要

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Technological problems in cultivation of some plant cells at high density were investigated. There were two main problems the difficulty of oxygen supply in cultivation of plant cells at high densities, and the bad effects of hydrodynamic stress generated by aeration-agitation operations used in supplying oxygen to the plant cells. To grasp the two problems quantitatively, two new intensity indices, i.e. an indensity index of oxygen supply in broth and an intensity index of hydrodynamic stress effects on plant cells, were proposed. Both indices were used in model systems with substitutes for plant cells made of granulated agar and β-naphthol particles.The characteristics of various bioreactors were investigated by the two indices and an image of a suitable bioreactor for culturing plant cells at high densities was found. In practice, using a jar fermenter (working volums 5 l) with a modifies paddle-type impeller and without baffle plates that was one of the suitable bioreactors, cultivation of plant cells at a high density (30 g dry weight per liter of medium) could be done.
公益社団法人日本生物工学会の論文
1985-05-25