ニホンナシ黒星病菌分生子懸濁液の効率的濃縮法

概要

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It is difficult to obtain concentrated conidial suspension of Japanese pear scab fungus, even after centrifugation, because of the conidial nature of the fungus which strongly tend to float in water. Several experiments were conducted to improve the method for preparing concentrated conidial suspension. When 10 ml of conidial suspension was added with small amount of diluted agar solution such as 0.5 ml of 0.1 % solution, and then centrifuged for more than five mimutes at 650×g, conidia were efficiently trapped in a portion of agar solution at the bottom of a tube. In this case, the efficiency of concetration of a conidial suspension was 41.0-fold compared with 50-fold of the theoretical value. And, in case of addition of 1 ml of 0.1% agar solution, it was 22.1-fold compared with 25-fold of the theoretical value. A concentration of conidia in rain water flowed down along twigs of Japanese pear tree is usually very low. Therefore, it is almost impossible to count the number of conidia in rain water directly under an optical microscope, however, the above mentioned concentration method made it possible. Diluted agar solution such as 0.1% is reserved in a refrigerator without antisepsis during six months free from degeneration and contamination of microorganisms. The method was named "agar solution concentration method", abbreviated to ASC method.
日本植物病理学会の論文
1989-07-25