Mesenchymal Stem Cells Differentiate into Tenocytes by Bone Morphogenetic Protein (BMP) 12 Gene Transfer(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Rhesus bone marrow mesenchymal stem cells (MSCs) were transfected with the BMP12 gene by electroporation, and the phenotype of the transfected cells was identified by morphological observation and molecular biological assay. After transfection, cells became slender, and their processes became thinner and were interwoven into a network. There were more organelles in the transfected cells than in the parental MSCs. The transfected cells exhibited mRNA expressions of BMP12, collagen type I and scleraxis, but not collagen type III mRNA expression. Immunocytochemical analysis also showed the presence of collagen type I, but not collagen type III in the transfected cells. The transfected cells were positive for CD44 and negative for HLA-DR. Therefore, MSCs can be introduced to differentiate into tenocytes by BMP12 gene transfection, and bone marrow MSCs can serve as an alternative seed cell for application in tendon tissue engineering.
- 社団法人日本生物工学会の論文
- 2005-10-25
著者
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Wang Qi-Wei
Department of Cell Engineering, Beijing Institute of Biotechnology
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Wang Qu-wei
Department Of Cell Engineering Beijing Institute Of Biotechnology
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Wang Qi-wei
Dep. Of Cell Engineering Beijing Inst. Of Biotechnology 20 Dongdajie Street Fengtai Beijing 100071 C
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Chen Zhao-lie
Dep. Of Cell Engineering Beijing Inst. Of Biotechnology 20 Dongdajie Street Fengtai Beijing 100071 C
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Chen Zhao-lie
Department Of Cell Engineering Beijing Institute Of Biotechnology
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Chen Zhao
Department Of Cell Engineering Institute Of Biotechnology
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Piao Ying
Department Of Computer Science And Department Of Central Laboratory The First Medical College Of Pla
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Piao Ying
Department Of Anatomy Faculty Of Medicine Kyoto University
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Piao Ying
Department Of Histology And Embryology Nanfang Medical University
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Wang Qi
Department Of Cell Engineering Beijing Institute Of Biotechnology
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Wang Qi
Department Of Agricultural And Biological Engineering University Of Illinois At Urbana-champaign
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PIAO Ying-Jie
Department of Anatomy , The First Medical University of PLA
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