Temperature Shift as a Process Optimization Step for the Production of Pro-urokinase by a Recombinant Chinese Hamster Ovary Cell Line in High-Density Perfusion Culture (BIOCHEMICAL ENGINEERING)

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Based on the effects of temperature shift on the cell cycle, apoptosis and metabolism of a recombinant Chinese hamster ovary (rCHO) cell line (CL-11G) producing pro-urokinase (pro-UK) in batch cultures, the potential of temperature shift as a tool in the optimization of the perfusion culture of CL-11G cells for the production of pro-UK was examined. The proportion of CL-11G cells in the G_0/G_l phase in static cultures increased from 56.4% to 82.8% following a temperature shift from 37℃ to 31℃. Conversely, the proportion of CL-11G cells in the S phase decreased from 34.8% to 11.6%. The specific growth rate of CL-11G cells reflected the effect of temperature on the cell cycle and decreased from 0.024 h^<-1> at 37℃ to 0.006 h^<-1> at 31℃. Continuous exposure to the non-permissive temperature of 31℃ led to a marginal increase in apoptosis. The specific pro-UK productivity of CL-11G cells increased by 74% at 34℃ compared with controls at 37℃ in batch cultures. CL-11G cells immobilized with Cytopore 1 in a 5-l bioreactor initiated at 37℃ and temperature shifted to 34℃ exhibited an average 17% increase in viable cell density and an average 47% increase in pro-UK production. These results demonstrated that temperature shift offers the prospect of enhancing the productivity of pro-UK in high-density perfusion culture.
社団法人日本生物工学会の論文
2004-04-25