Insulinoma Cell Line (MIN6) Adhesion and Spreading Mediated by Arg-Gly-Asp (RGD) Sequence Conjugated in Thermo-Reversible Gel(Cell and Tissue Engineering)

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概要

• 論文の詳細を見る

• We have functionalized gels with a putative cell-binding (-Arg-Gly-Asp-)(RGD) domain in an effort to regulate mammalian cell behavior in cells entrapped with gel. Adhesion molecules composed of Gly-Arg-Gly-Asp-Ser (GRGDS) peptides and cell recognition ligands were inculcated into thermo-reversible hydrogel composed of N-isopropylacrylamide, with a small amount of succinyl poly(ethylene glycol)(PEG) acrylate (MW 2000) used as a biomimetic extracellular matrix (ECM). The GRGDS-containing p(NiPAAm-co-PEG) copolymer gel was studied in vitro for its ability to promote cell spreading and to increase the viability of cells by introducing PEG spacers. Hydrogel lacking the adhesion molecules proved to be a poor ECM for adhesion, permitting only a 20% spread of the seeded cells after 10d. When PEG spacer arms, immobilized by a peptide linkage, had been integrated into the hydrogel, conjugation of RGD promoted cell spread by 300% in a 28-d trial. In addition, in a serum-free medium, only GRGDS peptides conjugated with the spacer arm were able to promote cell spread.

• 社団法人日本生物工学会の論文
• 2005-06-25

著者

• Na Kun

  Division Of Biotechnology The Catholic University Of Korea

• Park Keun

  College Of Medicine Pochon Cha University Cell And Gene Therapy Research Institute

• Kim Sung

  Biomaterials Research Center Korea Institute Of Science And Technology

• CHUNG HYUNG

  College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute 605

• Park Keun-hong

  College Of Medicine Pochon Cha University Cell And Gene Therapy Research Institute

• Chung Hyung

  College Of Medicine Pochon Cha University Cell And Gene Therapy Research Institute

• JUNG SEUNG

  College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute

• PARK KYU

  College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute

• CHA KWANG

  College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute

• Cha Kwang

  College Of Medicine Pochon Cha University Cell And Gene Therapy Research Institute

• Jung Seung

  College Of Medicine Pochon Cha University Cell And Gene Therapy Research Institute

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