Morphology of Spheroidal Hepatocytes within Injectable, Biodegradable, and Thermosensitive Poly (organophosphazene) Hydrogel as Cell Delivery Vehicle(CELL AND TISSUE ENGINEERING)

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概要

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• An aggregate of specific cells is often regarded as a better form of single-cell in artificial organs and mammalian cell bioreactors for improved cell-specific functions. In this study, freshly harvested primary rat hepatocytes, cultivated as spheroids and entrapped in a synthetic thermoreversible extracellular matrix, were examined for differentiation morphology and enhanced liver-specific functions and compared with a control set (single-cell hepatocytes). A copolymer of poly (organophsophazene) hydrogel, an injectable, biodegradable, and thermosensitive matrix, was used to entrap hepatocytes as spheroids or single cells. In a 28-d culture period, the spheroidal hepatocytes maintained a higher viability and produced albumin and urea at constant rates, whereas the single hepatocytes showed a reduction in the level of albumin secretion in the hydrogel. The culturing of spheroidal hepatocytes is a potentially useful three-dimensional cell system for application in bioartificial liver devices and bioreactors.

• 社団法人日本生物工学会の論文
• 2006-03-25

著者

• Park Keun-hong

  College Of Medicine Pochon Cha University Cha Stem Cell Institute

• Park Keun-hong

  College Of Medicine Pochon Cha University Cell And Gene Therapy Research Institute 605

• Song Soo

  Life Science Division Korea Institute Of Science And Technology

• Park Keun-hong

  College Of Medicine Pochon Cha University Cell And Gene Therapy Research Institute

関連論文

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