Cloning and Overexpression of the avi2 Gene Encoding a Major Cellulase Produced by Humicola insolens FERM BP-5977(Biochemistry & Molecular Biology)
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概要
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The avi2 gene encoding Avi2, which is a major cellulase produced by Humicola insolens FERM BP-5977, was cloned and sequenced. Avi2 showed high homology with other family 6 cellulases. The expression vector pNCE4 containing the avi2 gene was constructed, and this strain was transformed using a protoplast method. As a result, the pNCE4 transformant secreted 8-fold more Avi2 than the recipient strain.
- 社団法人日本農芸化学会の論文
- 2003-06-23
著者
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Murakami T
Microbiological Resources And Technology Laboratories Meiji Seika Kaisha Ltd.
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Watanabe M
Microbiological Resources And Technology Laboratories Meiji Seika Kaisha Ltd.
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MURAKAMI TAKESHI
Microbiological Resources and Technology Laboratories, Meiji Seika Kaisha, Ltd.
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WATANABE Manabu
Microbiological Resources and Technology Laboratories, Meiji Seika Kaisha, Ltd.
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SUMIDA Naomi
Microbiological Resources and Technology Laboratories, Meiji Seika Kaisha, Ltd.
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MORIYA Tatsuki
Microbiological Resources and Technology Laboratories, Meiji Seika Kaisya, Ltd.
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OKAKURA Kaoru
Microbiological Resources and Technology Laboratories, Meiji Seika Kaisya, Ltd.
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Sumida Naomi
Microbiological Resources And Technology Laboratories Meiji Seika Kaisha Ltd.
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Okakura Kaoru
Microbiological Resources And Technology Laboratories Meiji Seika Kaisya Ltd.
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Moriya Tatsuki
Microbiological Resources And Technology Laboratories Meiji Seika Kaisya Ltd.
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Murakami Takeshi
Microbiological Resources And Technology Laboratories Meiji Seika Kaisha Ltd.
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Morita Tatsuki
Microbiological Resources and Technology Laboratories, Meiji Seika Kaisya, Ltd.
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