Nucleotide Sequence of the Gene for a Thermostable Esterase from Pseudomonas putida MR-2068

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概要

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• The esterase gene (est) of Pseudomonas putida MR-2068 was cloned into Escherichia coli JM109. An 8-kb inserted DNA directed synthesis of an esterase in E. coli. The esterase gene was in a 1.1-kb PstI-ClaI fragment within the insert DNA. The complete nucleotides of the DNA fragment containing the esterase gene were sequenced and found to include a single open reading frame of 828bp coding for a protein of 276 amino acid residues. The open reading frame was confirmed by N-terminal amino acid sequence analysis of the purified esterase. A potential Shine-Dalgarno sequence is followed by the open reading frame. The esterase activity of the recombinant E. coli was more than 200 times higher than that of parental strain, P. putida MR-2068.

• 社団法人日本農芸化学会の論文
• 1995-07-23

著者

• Sakimae Akihiro

  Central Research Laboratories, Mitsubishi Rayon Co. Ltd.

• Numazawa Ryozo

  Head Office Mitsubishi Rayon Co. Ltd.

• Ozaki Eiji

  Central Research Laboratories, Mitsubishi Rayon Co., Ltd.

• Sakimae A

  Mitsubishi Rayon Co. Ltd. Hiroshima Jpn

• Ozaki E

  Univ. Tokyo Tokyo Jpn

関連論文

• Chemical Racemization of Methyl L-β-Acetylthioisobutyrate
• Nucleotide Sequence of the Gene for a Thermostable Esterase from Pseudomonas putida MR-2068
• Cloning and Expression of Pseudomonas putida Esterase Gene in Escherichia coli and Its Use in Enzymatic Production of D-β-Acetylthioisobutyric Acid
• Process Conditions for Production of D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate with the Cells of Pseudomonas putida MR-2068
• A Newly Isolated Microorganism Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate
• Screening of Microorganisms Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate

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