Cloning and Expression of Pseudomonas putida Esterase Gene in Escherichia coli and Its Use in Enzymatic Production of D-β-Acetylthioisobutyric Acid

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概要

• 論文の詳細を見る

• The esterase catalyzes the stereoselective hydrolysis of methyl DL-β-acetylthioisobutyrate (DL-ester) to give D-β-acetylthioisobutyric acid (DAT). To use the enzyme for DAT production, the esterasegene of Pseudomonas putida was cloned and expressed in E. coli. The recombinant E. coli containing the esterase gene produced a large amount of the enzyme in an active form. This strain could be used for the asymmetric synthesis of DAT.

• 社団法人日本農芸化学会の論文
• 1994-09-23

著者

• Sakimae Akihiro

  Central Research Laboratories, Mitsubishi Rayon Co. Ltd.

• Numazawa Ryozo

  Head Office Mitsubishi Rayon Co. Ltd.

• Ozaki Eiji

  Central Research Laboratories, Mitsubishi Rayon Co., Ltd.

• Numazawa Ryozo

  .Product Development Center, Mitsubishi Rayon Co., Ltd.

• Sakimae A

  Mitsubishi Rayon Co. Ltd. Hiroshima Jpn

• Ozaki E

  Univ. Tokyo Tokyo Jpn

関連論文

• Chemical Racemization of Methyl L-β-Acetylthioisobutyrate
• Nucleotide Sequence of the Gene for a Thermostable Esterase from Pseudomonas putida MR-2068
• Cloning and Expression of Pseudomonas putida Esterase Gene in Escherichia coli and Its Use in Enzymatic Production of D-β-Acetylthioisobutyric Acid
• Process Conditions for Production of D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate with the Cells of Pseudomonas putida MR-2068
• A Newly Isolated Microorganism Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate
• Screening of Microorganisms Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate

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