Process Conditions for Production of D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate with the Cells of Pseudomonas putida MR-2068
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概要
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The process conditions concerned with the production of D-β-acetylthioisobutyric acid were investigated. Methyl DL-β-acetylthioisobutyrate as an enzyme substrate was produced from methyl methacrylate in 96.6% yield by allowing 1.0 mol of methyl methacrylate to react with 1.6 mol of thioacetic acid at 80℃ for 6 h. Through the study of hydrolytic reaction of methyl DL-β-acetylthioisobutyrate with the cells of Pseudomonas putida MR-2068, it was found that D-β-acetylthioisobutyric acid having more than 98% (e.e.) optical purity was obtained when the reaction was done below pH 7.5 and below 50℃. For example, 10% (w/v) methyl DL-β-acetylthioisobutyrate in the reaction mixture was asymmetrically hydrolyzed by 0.2% (w/v) cells at 45℃ at pH 7.0 for 18 h to give DAT having 98.2% (e.e.) optical purity in 49.7% yield. To prevent the decompositon and racemization of D-β-acetylthioisobutyric acid in the purification process, the use of a thin-film distillation apparatus for a continuous distillation was proposed to be an effective purification method.
- 社団法人日本農芸化学会の論文
- 1993-05-23
著者
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Sakimae Akihiro
Central Research Laboratories, Mitsubishi Rayon Co. Ltd.
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Ohsuga Naoto
Central Research Laboratories, Mitsubishi Rayon Co. Ltd.
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Numazawa Ryozo
Central Research Laboratories, Mitsubishi Rayon Co. Ltd.
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Ohnishi Hisao
Central Research Laboratories, Mitsubishi Rayon Co. Ltd.
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Ohsuga Naoto
Central Research Laboratories Mitsubishi Rayon Co. Ltd.
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Ohnishi H
Kinki Univ. Nara Jpn
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Numazawa Ryozo
Head Office Mitsubishi Rayon Co. Ltd.
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Ozaki Eiji
Central Research Laboratories, Mitsubishi Rayon Co., Ltd.
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Toyama Hiroko
Central Research Laboratories, Mitsubishi Rayon Co., Ltd.
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Muraoka Itsumi
Central Research Laboratories, Mitsubishi Rayon Co., Ltd.
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Hamada Eiichi
Central Research Laboratories, Mitsubishi Rayon Co., Ltd.
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Sakimae A
Mitsubishi Rayon Co. Ltd. Hiroshima Jpn
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Hamada Eiichi
Central Research Laboratories Mitsubishi Rayon Co. Ltd.
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Muraoka Itsumi
Central Research Laboratories Mitsubishi Rayon Co. Ltd.
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Ozaki E
Univ. Tokyo Tokyo Jpn
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Toyama Hiroko
Central Research Laboratories Mitsubishi Rayon Co. Ltd.
関連論文
- Chemical Racemization of Methyl L-β-Acetylthioisobutyrate
- Microbial Production of Optically Active 1,3-Butanediol from the Racemate
- Microbial Production of Optically Active 1,3-Butanediol from 4-Hydroxy-2-butanone
- Nucleotide Sequence of the Gene for a Thermostable Esterase from Pseudomonas putida MR-2068
- Cloning and Expression of Pseudomonas putida Esterase Gene in Escherichia coli and Its Use in Enzymatic Production of D-β-Acetylthioisobutyric Acid
- Process Conditions for Production of D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate with the Cells of Pseudomonas putida MR-2068
- A Newly Isolated Microorganism Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate
- Screening of Microorganisms Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate