Growth-substrate Relationship of Tobacco Cells in Suspension Culture : Biomass Production of Tobacco Cells (VI)

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概要

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• Growth-substrate relationships in two sugar regimes (glucose and fructose) were examined in suspension cultures of tobacco cells in terms of the empirical equation proposed by Monod. A plot of 1/μ against 1/S for glucose concentration showed a linear correlation, giving μ_<max>=0.91 day^<-1> and K_s=0.25 g/l. Similarly, for fructose consumption, μ_<max>=0.92 day^<-1> and K_s=0.94 g/l were obtained for tobacco cell suspension culture. But in fructose medium, cellular growth at an initial fructose concentration of more than 10 g/l was often markedly inhibited, suggesting substrate inhibition. In addition, consumption of glucose and fructose in the medium containing both sugars as carbon sources is discussed.

• 公益社団法人日本生物工学会の論文
• 1981-02-25

著者

• Tsuji Kazumasa

  Central Research Institute The Japan Tobacco And Salt Public Corporation

• Kato Akira

  Central Research Institute The Japan Tobacco And Salt Public Corporation

関連論文

• Requirements of PO_4^, NO_3^-, SO_4^, K^+, and Ca^ for the growth of Tabacco Cells in Suspension Culture : Biomass Production of Tobacco Cells(III)
• Biomass Production of Nitrogen-reduced Tobacco Cells in Two-stage Continuous Culture : Biomass Producito of Tobacco Cells(V)
• Growth-substrate Relationship of Tobacco Cells in Suspension Culture : Biomass Production of Tobacco Cells (VI)
• Viscosity of the Broth of Tobacco Cells in Suspension Culture : Biomass Production of Tobacco Cells (Part IV)

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