Post-Transcriptional Regulation of Immunomodulatory Cytokines Production in Human Skin Fibroblasts by Intense Mechanical Stresses
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概要
- 論文の詳細を見る
We found that extremely high hydrostatic pressure stresses induced a variety of cytokines production in normal human dermal fibroblasts. Normal human dermal fibroblasts were found to survive and were active in producing interleukin (IL) -6, -8, and monocyte chemoattractant protein-1 (MCP-1) under extremely high hydrostatic pressure, up to 70 MPa (=690.8 atm= 713.8 kgf/cm^2). 70 MPa pressure application extremely enhanced IL-6 and IL-8 secretions (about 130 folds) without transcriptional enhancement. Although induction of IL-1α, IL-1β, and IL-12 mRNAs was appeared under high hydrostatic pressure condition, no translation of IL-1α, IL-1β, and IL-12 proteins was found. Extracellular accumulation of constitutively produced MCP-1 was down regulated in the pressure-applied fibroblasts in the absence of transcriptional repression. These results indicated that hydrostatic pressure stresses triggered post-transcriptional regulation mechanisms that modulated the cytokines production.
- 社団法人日本生物工学会の論文
- 2002-02-25
著者
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Aizawa M
Department Of Biological Information Graduate School Of Bioscience And Biotechnology Tokyo Institute
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Aizawa Masuo
Department Of Bioengineering Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Koyama Sumihiro
Frontier Research Program For Deep-sea Extremophiles Japan Marine Science And Technology Center
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FUJII SHINSUKE
Department of Genetics, Duke University Medical Center
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Fujii Shinsuke
Department Of Genetics Duke University Medical Center
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Fujii Shinsuke
Department Of Applied Biological Science Tokyo University Of Science
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