Improved Immobilization Yields by Addition of Protecting Agents in Glutaraldehyde-Induced Immobilization of Protease
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概要
- 論文の詳細を見る
The effects of peptides and polyethylene glycol (PEG) on the immobilization of protease using glutaraldehyde-activated Lewatit R258-K resin were investigated. When soy peptide was added at 0.1 g/l, the activity recovery and effectiveness factor increased by 20% and 38%, respectively. When PEG4000(0.5 g/l) was used as a protecting agent, the activity recovery and effectiveness factor were improved by 35% and 60%, respectively, leading to an increased specific activity of the immobilized enzyme. The addition of PEG4000 is suggested to improve the cost-effectiveness for the production of immobilized enzymes.
- 社団法人日本生物工学会の論文
- 2000-04-25
著者
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Kim Eui
Department Of Molecular Medicine Osaka University Graduate School Of Medicine
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Kim Eui
Department Of Chemical Engineering The University Of Seoul
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CHAE HEE
Department of Food Technology, Hoseo University
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IN MAN-JIN
Department of Human Nutrition and Food Science, Chungwoon University
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Chae Hee
Department Of Food Technology Hoseo University
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In Man-jin
Department Of Human Nutrition And Food Science Chungwoon University
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Chae Hee
Department Of Chemistry Education Seoul National University
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