Steady-State Oxidation Model by Horseradish Peroxidase for the Estimation of the Non-Inactivation Zone in the Enzymatic Removal of Pentachlorophenol
スポンサーリンク
概要
- 論文の詳細を見る
A theoretical model for the rate of oxidation of pentachlorophenol (PCP) catalyzed by horseradish peroxidase (HRP), was investigated to account for the influence of hydrogen peroxide (H_2O_2) concentration on the catalytic activity. To evaluate the maximum allowable H_2O_2 concentration, a relatively simple steady-state model was developed based on the Ping-Pong Bi-Bi mechanism considering the effect of excess H_2O_2. Several sets of experimental data obtained from batch reactions using an equimolar concentration of H_2O_2 and PCP were used to estimate the kinetic parameters by a nonlinear regression method. The model profiles acquired using the estimated parameters were in good agreement with experimental data at different initial enzyme and substrate concentrations. The best-fitted parameters were used to predict the initial rate of the enzyme reaction. The model prediction was coincident with the experimental results of other studies, indicating that the proposed model could be used for the optimization of reaction conditions. The maximum allowable H_2O_2 concentration to prevent H_2O_2 inhibition was calculated from the proposed model equation: [H_2O_2]_<0, max>=√(K^<H_2O_>_mK_1[PCP]_0)/(K^<PCP>_m+[PCP]_0). Using this equation, a curve depicting the non-inactivation zone for the two substrates (hydrogen peroxide and PCP) was plotted and it could be used for experimental design and optimal process operation. To minimize enzyme inactivation by H_2O_2, it was determined that the concentration of H_2O_2 should be lower than 2.78 mM, regardless of the stoichiometric ratio.
- 社団法人日本生物工学会の論文
- 1999-10-25
著者
-
Kim Eui
Department Of Molecular Medicine Osaka University Graduate School Of Medicine
-
Kim E
Univ. Seoul Seoul Kor
-
CHOI YU-JIN
Department of Chemical Engineering, University of Seoul
-
CHAE HEE
Center for Agricultural Biotechnology and Department of Chemical Engineering, University of Maryland
-
Kim Eui
Department Of Chemical Engineering The University Of Seoul
-
Chae H
Hoseo Univ. Asan Kor
-
Chae Hee
Center For Agricultural Biotechnology And Department Of Chemical Engineering University Of Maryland
-
Choi Yu-jin
Department Of Chemical Engineering University Of Seoul
-
Kim Eui
Department Of Chemical Engineering University Of Seoul
関連論文
- Successful treatment of relapsed T-cell non-Hodgkin's lymphoma with allogeneic peripheral blood stem cell transplantation with double conditioning
- Different Kinetics of WT1 and PML-RARα Gene Expression Levels during Remission Induction Therapy with All-trans Retinoic Acid Alone in Acute Promyelocytic Leukemia
- Monitoring Minimal Residual Disease in Leukemia Using Real-time Quantitative Polymerase Chain Reaction for Wilms Tumor Gene (WT1)
- Expression of the Wilms' Tumor Gene WT1 in Solid Tumors and Its Involvement in Tumor Cell Growth
- Inhibitory Effects of Saururus chinensis (LOUR.) BAILL on the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice(Pharmacology)
- Unmanipulated Reduced-Intensity Stem Cell Transplantation from a Haploidentical Donor Mismatched at 3 HLA Antigens to a Patient with Leukemic Transformation of Myelodysplastic Syndrome : Successful Second Transplantation after Graft Rejection
- Steady-State Oxidation Model by Horseradish Peroxidase for the Estimation of the Non-Inactivation Zone in the Enzymatic Removal of Pentachlorophenol
- Production of Cephalosporin C in a Fluidized-Bed Bioreactor
- Improved Immobilization Yields by Addition of Protecting Agents in Glutaraldehyde-Induced Immobilization of Protease
- Redifferentiation Therapy with 13-cis Retinoic Acids in Radioiodine-Resistant Thyroid Cancer
- Influence of hydration status in normal subjects : fractional analysis of parameters of Tc-99m DTPA and Tc-99m MAG_3 renography