Factors Affecting the Biodegradation of Cyanide by Burkholderia cepacia Strain C-3

概要

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The utilization of cyanide as a nitrogen source for growth in a liquid minimal medium by Burkholderia cepacia strain C-3 isolated from soil was demonstrated. The effects of various paramenters on the biodegradation of cyanide by the strain were investigated. Growth at the expense of cyanide occurred over a pH range from 8 to 10, and was optimal at pH 10. Growth and cyanide biodegradation were optimal at 30℃. The presence of Cu^<2+> or Fe^<2+> in the liquid medium at a concentration of 1mM inhibited both the growth of the bacterium and its cyanide degradation. The effects of Ni^<2+> and Co^<2+> and to a lesser extent Mn^<2+> and Mo^<2+> on cyanide degradation rate were concentration dependent. Addition of the cyanide-containing waste contaminants ethanol and methanol reduced the cyanide utilization of the isolate, but phenol was completely inhibitory. Microbial depletion of cyanide occurred even in the presence of other organic and inorganic nitrogen sources. Fructose, glucose, and mannose were the preferred carbon sources for cyanide biodegradation. The highest rate of cyanide degradation by β.cepacia C-3, 1.85 mag Cn h^<-1> was observed with 0.25%(w/v) fructose ; the degradation rate decreased progressively thereafter with increasing fructose concentration.
公益社団法人日本生物工学会の論文
2000-03-25