Effective Production of Amanitins by Two-Step Cultivation of the Basidiomycete, Galerina fasciculata GF-060
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概要
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Practical production of amanitins was attempted by fermentation using a basidiomycete, Galerina fasciculata GF-060. In liquid fermentation, intracellular α- and γ-amanitins were the main products, while α- and β-amanitins accumulated in solid cultured mycelia. The production of amanitins in liquid fermentation was strongly affected by the amount of the remaining carbon sources (particularly glucose and sucrose). In batch cultivation, the productivity of α-amanitin was 1.58 mg/l. To improve the productivity, replacement cultivation using glucose-free medium was attempted. As a result, the maximum production of α-amanitin reached 5.02 mg/l. These conditions (fermentation style and glucose starvation) are effective for the production of all the known types of amanitins.
- 公益社団法人日本生物工学会の論文
- 2000-01-25
著者
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Shinozawa Takao
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University
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MURAOKA SHINJIRO
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University
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Muraoka Shinjiro
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University:research A
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