Production of Optically Active Ketoprofen by Direct Enzymatic Esterification

概要

論文の詳細を見る
For the production of optically active ketoprofen, enzymatic resolution of recemic ketoprofen in a organic solvent has been accomplished via enantioselective esterification. Pharmacologically inactive(R)-ketoprofen is converted into the corresponding(R)-ester by this method. Enantioselectivity in lipase-catalyzed resolution of recemic ketoprofen was mainly dependent on the sources of lipase, alcohol moiety, organic solvent, and water content. Ethanol was used as the alkyl donor and the optimum water content required for highly efficient enzymatic resolution was determined to be 0.1-0.15%(v/v), which was maintained using salt hydrates such as Na_2SO_4・10H_2O. (S)-Ketoprofen could be obtained with high enantioselectivity(E=15)in n-hexane supplemented with ethylene dichloride(20%(v/v))using commericially available Candida antarctica lipase(Novozym^[○R!]435).
社団法人日本生物工学会の論文
1999-04-25