Overproduction of Human Granulocyte-Colony Stimulating Factor Fused to the PelB Signal Peptide in Escherichia coli

概要

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In an attempt to induce the secretion of human granulocyte-colony stimulating factor(hG-CSF) in Escherichia coli, the gene encoding mature hG-CSF was fused to the gene for the pectate lyase B(pelB) signal peptide of Erwinia caratovora, and fed-batch fermentation of the recombinant E.coli was performed. No secretion of hG-CSF and no processing of the signal peptide from the fusion protein was observed. Instead, the fusion protein, pelB-hG-CSF, at a concentration of ca.1.7 g/l was produced in an inclusion body form. Interestingly, the biological activity of the purified fusion protein was found to be identical to that of mature hG-CSF, indicating that the pelB signal peptide attached to the N-terminal of hG-CSF had no effect on its biological activity.
社団法人日本生物工学会の論文
1998-04-25