Overproduction of Human Granulocyte-Colony Stimulating Factor Fused to the PelB Signal Peptide in Escherichia coli
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概要
- 論文の詳細を見る
In an attempt to induce the secretion of human granulocyte-colony stimulating factor(hG-CSF) in Escherichia coli, the gene encoding mature hG-CSF was fused to the gene for the pectate lyase B(pelB) signal peptide of Erwinia caratovora, and fed-batch fermentation of the recombinant E.coli was performed. No secretion of hG-CSF and no processing of the signal peptide from the fusion protein was observed. Instead, the fusion protein, pelB-hG-CSF, at a concentration of ca.1.7 g/l was produced in an inclusion body form. Interestingly, the biological activity of the purified fusion protein was found to be identical to that of mature hG-CSF, indicating that the pelB signal peptide attached to the N-terminal of hG-CSF had no effect on its biological activity.
- 社団法人日本生物工学会の論文
- 1998-04-25
著者
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Yu Mi
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu Unive
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KIM Beom
Department of Dermatology, Seoul National University College of Medicine
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Lee Young
Korea Food Research Institute
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Chung Bong
Korea Research Institute Of Bioscience And Biotechnology
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Sohn Mi-jin
Korea Research Institute Of Bioscience And Biotechnology
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Oh Su-wan
Korea Research Institute Of Bioscience And Biotechnology
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Kim Beom
Department Of Chemical Engineering Konyang University
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Lee Young
Korea Advanced Institute Of Science And Technology
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PARK UI-SUN
Korea Research Institute of Bioscience and Biotechnology
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POO HARYOUNG
Korea Research Institute of Bioscience and Biotechnology
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Yu Mi
Department Of Chemical Engineering Konyang University
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Lee Young
Korea Research Institute Of Bioscience And Biotechnology
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