Immobilization of Cyclodextrin Glucanotranferase on Capillary Membrane

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概要

• 論文の詳細を見る

• A bioreactor system with the enzyme immobilized on a capillary memberane is a promising tool for the mass production of valuable substnces, because of the good prtoductive efficiency. To investigate the kinetics of immobilized cyclodextrin glucanotransferase ([EC 2.4.1.19]; CGTase) on a capillary membrane in a bioreactor system, the amount of immobilized CGTase and the operating conditions, such as pressure and the reaction temperature, were examined under a constant substrate concentration (1.0%) and a constant flow rate (0.12 m/s). When the CGTase was immobilized at a concentration of 0.04 to 0.62 mg per membrane area(cm^2), the decrease in the immobilized amont of CGTase resulted in an increase in the cyclodextrin production rate (g of CD/h・m^2; CPR) and the CPR correlated well with the flux of the CGTase-immobilized membrane. Althrough a higher reaction temperature caused an increase in the CPR within a short operating time of the bioreactor, repeated operation at 60℃ led to a reduction in the CPR due to the denaturation of the immobilized CGTase. The percentage of cyclodextrin (CD) to total sigar obtained in the permeate was slightrly more than 60% under most operating conditions, but immobilization of the exces amount of CGTase (0.42-0.62 mg/cm^2) reduced the CD yield as well as the ratio of α-CD to β-CD, suggesting that it led to a CGTase side-reaction such as intermolecular transglycosylation. These data suggest that the conditions under which the bioreactor with 0.04-0.40 mg/cm^2 was operated; a reaction temperature of 50℃, a residence time of 1-2 min and adjustable pressure, could be employed to obtain a high CPR using a large scale CGTase-immopbilized membrane bioreactor.

• 社団法人日本生物工学会の論文
• 1994-03-25

著者

• Ito M

  Medical Research Laboratory. Nittro Deko Corporation

• Okada T

  Tsukuba Univ. Ibaraki Jpn

• OKADA TAKESHI

  Medical Research Laboratory, Nitto Denko Corporation

• ITO MASAAKI

  Medical Research Laboratory, Nitto Denko Corporation

• HIBINO KEN

  Medical Research Laboratory, Nitto Denko Corporation

• Hibino K

  Medical Research Laboratory Nitto Denko Corporation

関連論文

• Comparative Studies of β-1,3-Glucanase A1 and B of Bacillus circulans WL-12 : Purifications and Enzymatic Properities
• Structure of the Gene Encoding β-1,3-Glucanase B of Bacillus circulans WL-12
• Intermolecular Transglycosylating Reaction of Cyclodextrin Glucanotransferase Immobilized on Capillary Membrane
• Immobilization of Cyclodextrin Glucanotranferase on Capillary Membrane
• Breeding by Interspecific Protoplast Fusion of Koji-Molds, Aspergillus awamori var. kawachi and Aspergillus usamii mut. shirousamii

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