Simultaneous Measurement of the Galacturonate and Neutral Sugar Contents of Pectic Substances by an Enzymic-HPLC Method
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概要
- 論文の詳細を見る
An enzymic-HPLC method was successfully applied to the simultaneous analysis of the galacturonate and neutral sugar contents of pectic substances. A mixture of seven neutral sugars that are present in oridnary pectins was eluted as one peak on a Shodex SUGAR SH1821 column, using 0.001N sulfuric acid as the eluent ; the peak was completely separated from that of galacturonate. No difference in the peak-area ratios of individual neutral sugars to glycerol (internal standard) was found among the seven ; the relationship between the peak response and concentration was strictly linear throughout the entire concentration range studied. Seventeen pectic samples, including pectins, pectates, and rhamnogalacturonan fragments, were completely prehydrolyzed by Driselase, an industrial enzyme product from Irpex lacteus, and then analyzed for their constituent sugar contents. The enzymic-HPLC method was simple, accurate, and particularly useful for routine pectin analyses.
- 社団法人日本農芸化学会の論文
- 1992-07-23
著者
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Matsuhashi S
(present Address) Japan Atomic Energy Research Institute Takasaki Radiation Chemistry Research Estab
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HATANAKA Chitoshi
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University
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MATSUHASHI Shinpei
(Present address) Japan Atomic Energy Research Institute, Takasaki Radiation Chemistry Research Esta
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INOUE Shin-ichi
(Present address) Kuraray Co., Ltd.
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Hatanaka Chitoshi
Department Of Applied Biochemistry Faculty Of Applied Biological Science Hiroshima University
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Matsuhashi Shinpei
(present Address) Japan Atomic Energy Research Institute Takasaki Radiation Chemistry Research Estab
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Inoue Shin-ichi
(present Address) Kuraray Co. Ltd.
関連論文
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