Inhibition of Achromobacter Protease I by Lysinal Derivatives

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概要

• 論文の詳細を見る

• Z-Val-, Z-Pro-, Z-Leu-Leu-, and Z-Leu-Pro-lysinals and Bz-DL-lysinal were chemically synthesized and tested as novel inhibitors for Achromobacter protease I (API), a lysine-specific serine protease. Among the lysinal derivatives tested, Z-Val-lysinal was the most potent competitive inhibitor, its K_i being estimated as 6.5 nM in an esterolytic assay with Tos-Lys-OMe. In an amidolytic assay, Z-Leu-Leu-lysinal was the most potent inhibitor and the apparent mode of inhibition was non-competitive. The K_is of the other lysinal derivatives in both esterolytic and amidolytic assays were more than 10^3 times lower than that of leupeptin. Z-Val-lysinol, lacking the aldehyde group, was a poor competitive inhibitor. These results suggest that acyl-, aclyaminoacyl-, and acylpeptidyllysinals function as a transition-state inhibitor for Achromobacter protease I.

• 社団法人日本農芸化学会の論文
• 1992-10-23

著者

• Sakiyama F

  International Buddhist University

• Sakiyama Fumio

  Division Of Protein Chemistry Institute For Protein Research Osaka University

• Sakiyama Fumio

  Institute For Protein Research Osaka University

• Masaki Takeharu

  Department of Resource Biology, Faculty of Agriculture, Ibaraki University

• Tsunasawa Susumu

  Institute for Protein Research, Osaka University

• Soejima Masami

  Department of Resource Biology, Faculty of Agriculture, Ibaraki University

• TANAKA Takumi

  Osaka Research Laboratories, Wako Pure Chemical Industries, Ltd.

• Soejima Masami

  Department Of Resource Biology Faculty Of Agriculture Ibaraki University

• Masaki T

  Department Of Bioresource Science College Of Agriculture Ibaraki University

• Masaki Takeharu

  Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University

• Tanaka Takumi

  Osaka Research Laboratories Wako Pure Chemical Industries Ltd.

• Tsunasawa S

  Takara Shuzo Co. Ltd. Shiga

• Tsunasawa Susumu

  Institute For Protein Research Osaka University

• Soejima Masami

  Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University

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