Isolation of Mitogenic Glycophosphopeptides from Cheese Whey Protein Concentrate
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概要
- 論文の詳細を見る
We investigated the immunological function of cheede whey protein concentrate (CWPC), which is a by-product of cheese production, using mitogenic activity in murine splenocytes as an index. A fraction isolated by gel filtration and anion exchange chromatography of CWPC showed high mitogenic activity, comparable to the activity of lipopolysaccharide (LPS). The fraction was detected as a single band on SDS-PAGE. It contained calcium, inorganic phosphorus, and carbohydrate, indicating the active component to be a glycophosphopeptide (GPP). Since Pronase digestion of GPP did not reduce its mitogenic activity, carbohydrate rather than peptide may be important in the activity. When applied on an anti-β-caseinophosphopeptide (β-CPP) antibody aftinity column, the GPP was separated into two components, one with affinity to β-CPP and the other without such affinity. Both the components contained N-linked oligosaccharide chains and had the mitogenic activity. These results demonstrate that cheese whey contains a GPP having strong mitogenic activity.
- 社団法人日本農芸化学会の論文
- 1996-03-23
著者
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Yun S
Nihon Univ. Tokyo
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Kumagai Susumu
Department Of Biomedical Food Research National Institute Of Infectious Diseasea(former National Ins
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Kumagai S
Department Of Biomedical Food Research National Institute Of Health
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Sugita Konishi
Department Of Biomedical Food Research National Institute Of Health
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Yamauchi Kunio
The College Of Agriculture And Veterinary Medicine Nihon Univeisity
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Yun Sung
The College Of Agriculture And Veterinary Medicine Nihon University
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Kumagai Susumu
Department Of Biomedical Food Research
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