Purification and Characterization of a Di-D-fructofuranose 2',1;2,1'-dianhydride Producing Enzyme from Streptomyces sp. MCI-2524

概要

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An extracellular enzyme that produces di-D-fructofuranose 2',1;2,1'-dianhydride (difructose anhydride I=DFA I) from inulin was purified from the culture broth of Streptomyces sp. MCI-2524. The purification enhanced the specific activity 7-fold with an overall yield of 17%. The purified enzyme, when electrophoresed on a SDS polyacrylamide gel, gave a single band corresponding to a molecular weight of 36 kDa. Gel filtration chromatography gave a single peak that eluted at a position corresponding to 70 kDa. The enzyme was active from pH 3.0 to pH 9.0, and at temperatures up to 65℃. Maximal activity was observed at pH 6.0, at 55℃. The enzyme was inhibited by Cu^<2+>.
社団法人日本農芸化学会の論文
1993-12-23