Purification and Characterization of Copper-metallothioneins from Aspergillus niger

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概要

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• Copper metallothioneins were extracted from Aspergillus niger after an induction period of five hours in a copper-containing suspension medium. The crude extract (FI) was obtained by cell homogenization and the partially purified extract (FII) was obtained by heat treatment at 60℃ for 10 min. The partially purified extract (FII) was further purified by successive chromatographies on size-exclusion (Biogel P-30) and ion-exchange (TSK-HW 65-DEAE) chromatography columns. All protein fractions were analyzed for their protein and induced copper contents. The Cu/protein ratio increased steadily throughout the purification process ; the most purified fraction (FIV) showed a 24-fold increase, with a recovery of 13%. The molecular weight of the Cu-metallothionein fraction FIV was 11,000 and the ratio of moles of copper per mole of protein was 6.6.

• 社団法人日本農芸化学会の論文
• 1993-09-23

著者

• KERMASHA Selim

  Department of Food Science and Agricultural Chemistry, McGill University

• Kermasha Selim

  Department Of Food Science And Agricultural Chemistry Mcgill University

• Goetghebeur Mireille

  Department Of Food Science And Agricultural Chemistry Mcgill University

• Pellerin Frederic

  Department of Food Science and Agricultural Chemistry, McGill University

• Rovel Bernard

  Laboratoire de Biochimie Appliquee, Institut National Polytechnique de Lorraine

• Metche Maurice

  Laboratoire de Biochimie Appliquee, Institut National Polytechnique de Lorraine

• Rovel Bernard

  Laboratoire De Biochimie Appliquee Institut National Polytechnique De Lorraine

• Metche Maurice

  Laboratoire De Biochimie Appliquee Institut National Polytechnique De Lorraine

• Pellerin Frederic

  Department Of Food Science And Agricultural Chemistry Mcgill University

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• Purification and Characterization of Copper-metallothioneins from Aspergillus niger

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