New Parameters for Simulating Progress Curves of the Lipase Reaction

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概要

• 論文の詳細を見る

• Progress curves of the reactin of lipase from Pseudomonas fluorescens biotype I simulated a hyperbolic equation. The hydrolysis ratio γ at a reaction time t of the lipolysis was expressed by the equation γ=tR./(T_m+t). The double reciprocal plots of γ and t lay close to a straight line, and R and t_m could be evaluated from the co-ordinates of the straight line and the axes. The value R is the hydrolysis ratio up to a reaction time of infinity, t_m is the reaction time up to R/2 of the hydrolysis ratio. A comparison between the simulation and a scheme of the general hydrolysis process indicated that the simulation may apply to the general hydrolysis process. Studies of schemes of the enzyme reaction suggested that t_m is a function of the Michaelis constant, the maximum velocity, R, and the initial substrate concentration ; R is a function of the initial substrate concentration and the equilibrium constant if the amount of enzyme is large enough or the enzyme is stable enough for the active enzyme to exist until the reaction reaches equilbrium ; and R is a function of the initial enzyme concentration, the deactivation constant, and t_m, if the active enzyme is not able to exist up to the equilibrium.

• 公益社団法人日本生物工学会の論文
• 1983-06-25

著者

• SUZUKI Hideo

  Fermentation Research Institute

• Kosugi Yoshitsugu

  Fermentation Research Institute

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